Trypsin-protease activated receptor-2 signaling contributes to pancreatic cancer pain.

ABSTRACT: Pain treatment is a critical aspect of pancreatic cancer patient clinical care. This study investigated the role of trypsin-protease activated receptor-2 (PAR-2) in pancreatic cancer pain. Pancreatic tissue samples were collected from pancreatic cancer (n=22) and control patients (n=22). Immunofluorescence analyses confirmed colocalization of PAR-2 and neuronal markers in pancreatic cancer tissues. Trypsin levels and protease activities were higher in pancreatic cancer tissue specimens than in the controls. Supernatants from cultured human pancreatic cancer tissues (PC supernatants) induced substance P and calcitonin gene-related peptide release in dorsal root ganglia (DRG) neurons, and FS-NH₂, a selective PAR-2 antagonist, inhibited this effect. A BALB/c nude mouse orthotopic t

SUBMITTER: Zhu J 

PROVIDER: S-EPMC5617466 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature