Sheathless Microflow Cytometry Using Viscoelastic Fluids.

ABSTRACT: Microflow cytometry is a powerful technique for characterization of particles suspended in a solution. In this work, we present a microflow cytometer based on viscoelastic focusing. 3D single-line focusing of microparticles was achieved in a straight capillary using viscoelastic focusing which alleviated the need for sheath flow or any other actuation mechanism. Optical detection was performed by fiber coupled light source and photodetectors. Using this system, we present the detection of microparticles suspended in three different viscoelastic solutions. The rheological properties of the solutions were measured and used to assess the focusing performance both analytically and numerically. The results were verified experimentally, and it has been shown that polyethlyene oxide (PEO) and hya

SUBMITTER: Asghari M 

PROVIDER: S-EPMC5617843 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature