Bioreducible Hydrophobin-Stabilized Supraparticles for Selective Intracellular Release.

ABSTRACT: One of the main hurdles in nanomedicine is the low stability of drug-nanocarrier complexes as well as the drug delivery efficiency in the region-of-interest. Here, we describe the use of the film-forming protein hydrophobin HFBII to organize dodecanethiol-protected gold nanoparticles (NPs) into well-defined supraparticles (SPs). The obtained SPs are exceptionally stable in vivo and efficiently encapsulate hydrophobic drug molecules. The HFBII film prevents massive release of the encapsulated drug, which, instead, is activated by selective SP disassembly triggered intracellularly by glutathione reduction of the protein film. As a consequence, the therapeutic efficiency of an encapsulated anticancer drug is highly enhanced (2 orders of magnitude decrease in IC₅₀). Biodistribution

SUBMITTER: Maiolo D 

PROVIDER: S-EPMC5618140 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature