Stable Membrane-Association of mRNAs in Etiolated, Greening and Mature Plastids.

ABSTRACT: Chloroplast genes are transcribed as polycistronic precursor RNAs that give rise to a multitude of processing products down to monocistronic forms. Translation of these mRNAs is realized by bacterial type 70S ribosomes. A larger fraction of these ribosomes is attached to chloroplast membranes. This study analyzed transcriptome-wide distribution of plastid mRNAs between soluble and membrane fractions of purified plastids using microarray analyses and validating RNA gel blot hybridizations. To determine the impact of light on mRNA localization, we used etioplasts, greening plastids and mature chloroplasts from Zea mays as a source for membrane and soluble extracts. The results show that the three plastid types display an almost identical distribution of RNAs between the two organellar fracti

SUBMITTER: Legen J 

PROVIDER: S-EPMC5618530 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature