Hypoxic preconditioning-induced autophagy enhances survival of engrafted endothelial progenitor cells in ischaemic limb.

ABSTRACT: Recent clinical studies have suggested that endothelial progenitor cells (EPCs) transplantation provides a modest benefit for treatment of the ischaemic diseases such as limb ischaemia. However, cell-based therapies have been limited by poor survival of the engrafted cells. This investigation was designed to establish optimal hypoxia preconditioning and evaluate effects of hypoxic preconditioning-induced autophagy on survival of the engrafted EPCs. Autophagy of CD34⁺ VEGFR-2⁺ EPCs isolated from rat bone marrow increased after treatment with 1% O₂ . The number of the apoptotic cells in the hypoxic cells increased significantly after autophagy was inhibited with 3-methyladenine. According to balance of autophagy and apoptosis, treatment with 1% O₂

SUBMITTER: Zhou P 

PROVIDER: S-EPMC5618704 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature