Transcriptome analysis reveals differences in mechanisms regulating cessation of luteal function in pregnant and non-pregnant dogs.

ABSTRACT:

Background

In the domestic dog, corpora lutea (CL) are the only source of progesterone (P4), both in pregnant and non-pregnant cycles because there is no placental steroidogenesis. The absence of an endogenous luteolysin in absence of pregnancy results in long-lasting physiological pseudopregnancy, strongly contrasting with the acute luteolysis observed prepartum. The underlying biological mechanisms and the involvement of P4 signalling remain, however, not fully understood. Therefore, here, next-generation sequencing (RNA-Seq) was performed on CL from the late luteal phase and compared with normally luteolyzing CL collected at the prepartum P4 decrease.

Results

The contrast "luteal regression over luteolysis" yielded 1595 differentially expressed genes (DEG). The CL in late

SUBMITTER: Zatta S 

PROVIDER: S-EPMC5618722 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature