Targeted antigen delivery by an anti-class II MHC VHH elicits focused αMUC1(Tn) immunity.

ABSTRACT: Unusual patterns of glycosylation on the surface of transformed cells contribute to immune modulation and metastasis of malignant tumors. Active immunization against them requires effective antigen presentation, which is complicated by a lack of access to tumor-specific posttranslational modifications through standard genetic approaches and by the low efficiency of passive antigen sampling. We found that antigen targeted to antigen presenting cells via class II MHC products can elicit a robust immune response against MUC1(Tn) bearing a defined tumor-associated glycoform, Tn. The two-component vaccine construct was prepared by sortase-mediated protein ligation of a synthetic MUC1(Tn) fragment to a class II MHC-binding single-domain antibody fragment (VHH7) as targeting moiety. We sho

SUBMITTER: Fang T 

PROVIDER: S-EPMC5618788 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature