Structural Dynamics of 15-Lipoxygenase-2 via Hydrogen-Deuterium Exchange.

ABSTRACT: Eicosanoids are inflammatory signaling lipids that are biosynthesized in response to cellular injury or threat. They were originally thought to be pro-inflammatory molecules, but members of at least one subclass, the lipoxins, are able to resolve inflammation. One step in lipoxin synthesis is the oxygenation of arachidonic acid by 15-lipoxygenase (15-LOX). 15-LOX contains two domains: a Ca²⁺ binding PLAT domain and a catalytic domain. 15-LOX is a soluble cytosolic protein until binding of Ca²⁺ to the PLAT domain promotes translocation to the membrane surface. The role of 15-LOX structural dynamics in this translocation has remained unclear. We investigated the dynamics of 15-LOX isoform B (15-LOX-2) upon binding of Ca²⁺ and ligands, as well as upon membrane

SUBMITTER: Droege KD 

PROVIDER: S-EPMC5619234 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature