CO₂ assimilation strategies in stratified lakes: Diversity and distribution patterns of chemolithoautotrophs.

ABSTRACT: While mechanisms of different carbon dioxide (CO₂ ) assimilation pathways in chemolithoautotrohic prokaryotes are well understood for many isolates under laboratory conditions, the ecological significance of diverse CO₂ fixation strategies in the environment is mostly unexplored. Six stratified freshwater lakes were chosen to study the distribution and diversity of the Calvin-Benson-Bassham (CBB) cycle, the reductive tricarboxylic acid (rTCA) cycle, and the recently discovered archaeal 3-hydroxypropionate/4-hydroxybutyrate (HP/HB) pathway. Eleven primer sets were used to amplify and sequence genes coding for selected key enzymes in the three pathways. Whereas the CBB pathway with different forms of RubisCO (IA, IC and II) was ubiquitous and related to diverse bacteria

SUBMITTER: Alfreider A 

PROVIDER: S-EPMC5619642 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature