Optimizing the role of limbal explant size and source in determining the outcomes of limbal transplantation: An in vitro study.

ABSTRACT:

Purpose

Simple limbal epithelial transplantation (SLET) and cultivated limbal epithelial transplantation (CLET) are proven clinical techniques for treating limbal stem cell deficiency (LSCD). However, the ideal size and number of the limbal explants required for transplantation has not been clearly elucidated. This in vitro study aimed to determine the optimal limbal explant size required for complete corneal epithelialization by characterizing the cell expansion.

Methods

Limbal explants obtained from both live and cadaveric biopsies were cultured on the denuded amniotic membrane. Explant size and the explant cell outgrowth (expansion) were measured using ImageJ software with respect to days. Cultures were characterized by assessing the rate of proliferation of cells with 5-

SUBMITTER: Kethiri AR 

PROVIDER: S-EPMC5619808 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature