Progress in small-angle scattering from biological solutions at high-brilliance synchrotrons.

ABSTRACT: Small-angle X-ray scattering (SAXS) is an established technique that provides low-resolution structural information on macromolecular solutions. Recent decades have witnessed significant progress in both experimental facilities and in novel data-analysis approaches, making SAXS a mainstream method for structural biology. The technique is routinely applied to directly reconstruct low-resolution shapes of proteins and to generate atomistic models of macromolecular assemblies using hybrid approaches. Very importantly, SAXS is capable of yielding structural information on systems with size and conformational polydispersity, including highly flexible objects. In addition, utilizing high-flux synchrotron facilities, time-resolved SAXS allows analysis of kinetic processes over time ranges from mi

SUBMITTER: Tuukkanen AT 

PROVIDER: S-EPMC5619845 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature