Lambda phage-based vaccine induces antitumor immunity in hepatocellular carcinoma.

ABSTRACT:

Background and aims

Hepatocellular carcinoma (HCC) is a difficult to treat tumor with a poor prognosis. Aspartate β-hydroxylase (ASPH) is a highly conserved enzyme overexpressed on the cell surface of both murine and human HCC cells.

Methods

We evaluated therapeutic effects of nanoparticle lambda (λ) phage vaccine constructs against ASPH expressing murine liver tumors. Mice were immunized before and after subcutaneous implantation of a syngeneic BNL HCC cell line. Antitumor actively was assessed by generation of antigen specific cellular immune responses and the identification of tumor infiltrating lymphocytes.

Results

Prophylactic and therapeutic immunization significantly delayed HCC growth and progression. ASPH-antigen specific CD4+ and CD8+ lymphocytes were identi

SUBMITTER: Iwagami Y 

PROVIDER: S-EPMC5619992 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature