High-throughput RNA structure probing reveals critical folding events during early 60S ribosome assembly in yeast.

ABSTRACT: While the protein composition of various yeast 60S ribosomal subunit assembly intermediates has been studied in detail, little is known about ribosomal RNA (rRNA) structural rearrangements that take place during early 60S assembly steps. Using a high-throughput RNA structure probing method, we provide nucleotide resolution insights into rRNA structural rearrangements during nucleolar 60S assembly. Our results suggest that many rRNA-folding steps, such as folding of 5.8S rRNA, occur at a very specific stage of assembly, and propose that downstream nuclear assembly events can only continue once 5.8S folding has been completed. Our maps of nucleotide flexibility enable making predictions about the establishment of protein-rRNA interactions, providing intriguing insights into the temporal orde

SUBMITTER: Burlacu E 

PROVIDER: S-EPMC5620067 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature