Isolation and detection of circulating tumour cells from metastatic melanoma patients using a slanted spiral microfluidic device.

ABSTRACT: Circulating Tumour Cells (CTCs) are promising cancer biomarkers. Several methods have been developed to isolate CTCs from blood samples. However, the isolation of melanoma CTCs is very challenging as a result of their extraordinary heterogeneity, which has hindered their biological and clinical study. Thus, methods that isolate CTCs based on their physical properties, rather than surface marker expression, such as microfluidic devices, are greatly needed in melanoma. Here, we assessed the ability of the slanted spiral microfluidic device to isolate melanoma CTCs via label-free enrichment. We demonstrated that this device yields recovery rates of spiked melanoma cells of over 80% and 55%, after one or two rounds of enrichment, respectively. Concurrently, a two to three log reduction of whit

SUBMITTER: Aya-Bonilla CA 

PROVIDER: S-EPMC5620178 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature