Sperm-carried RNAs play critical roles in mouse embryonic development.

ABSTRACT: Recently, numerous studies have reported that the mature sperm contains both coding and non-coding RNAs and the sperm delivers some RNAs to the oocyte at fertilization. However, the functions of the RNAs carried to the oocyte by sperm at fertilization in embryonic development remains a mystery. In this study, the mature spermatozoa were treated with lysolecithin, pronase and RNases (RNase A and RNase H) to remove the sperm-carried RNAs, and then injected into normal mature oocyte. The results showed that after the treatment, the content of the sperm RNAs was decreased by about 90%. The blastocyst formation rate and the live birth rate of the embryos from intracytoplasmic sperm injection (ICSI) using the treated sperm were significantly decreased (P<0.01), while these effects were pa

SUBMITTER: Guo L 

PROVIDER: S-EPMC5620181 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature