Gene Scanning for Microdeletions in the Azoospermia Factor Region of Y-Chromosome in Infertile Men of Gujarat, India.

ABSTRACT:

Introduction

Azoospermia Factor (AZF) microdeletions in Yq chromosome is one of the most frequent genetic cause associated with failure of spermatogenesis in males with infertility.

Aim

To figure out the Yq chromosome microdeletions frequency in infertile men from Gujarat region of India.

Materials and methods

In this study, 141 infertile men with azoospermia (n=41) and oligozoospermia (n=100) were examined along with 159 normozoospermic men. Eleven different markers spanning the azoospermia factor region of human Yq chromosome, amplified by sequence-tagged site Polymerase Chain Reaction (PCR) to detect the microdeletions. Sperm morphological analysis was done using papanicolau staining method.

Results

Thirty four infertile men out of 141 presented Yq chromosom

SUBMITTER: Nailwal M 

PROVIDER: S-EPMC5620795 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature