Development and Validation of a Reproducible and Label-Free Surface Plasmon Resonance Immunosensor for Enrofloxacin Detection in Animal-Derived Foods.

ABSTRACT: This study describes the development of a reproducible and label-free surface plasmon resonance (SPR) immunosensor and its application in the detection of harmful enrofloxacin (ENRO) in animal-derived foods. The experimental parameters for the immunosensor construction and regeneration, including the pH value (4.5), concentration for coating ENRO-ovalbumin conjugate (ENRO-OVA) (100 μg·mL-1), concentration of anti-ENRO antibody (80 nM) and regeneration solution (0.1 mol·L-1 HCl) were evaluated in detail. With the optimized parameters, the proposed SPR immunosensor obtained a good linear response to ENRO with high sensitivity (IC50: 3.8 ng·mL-1) and low detection limit (IC15: 1.2 ng·mL-1). The proposed SPR immunosensor was further validated to have favorable performances for ENRO residue det

SUBMITTER: Pan M 

PROVIDER: S-EPMC5621032 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature