Immunoprotective potential of BamA, the outer membrane protein assembly factor, against MDR Acinetobacter baumannii.

ABSTRACT:

Abstarct

Acinetobacter baumannii infections are responsible for major health problems in immunocompromised patients particularly in intensive care units. Due to rapid acquisition of and also inherent drug resistance, a vaccine is an effective treatment option against this pathogen. BamA, an outer membrane β-barrel assembly protein, was identified in A. baumannii as potential vaccine candidate by in silico analysis. The immunoprotective efficacy of this highly conserved protein was investigated against a virulent multidrug resistant clinical isolate using murine pneumonia model. Recombinant BamA elicited a high IgG antibody titer (160000) in mice. Opsonophagocytic killing assay showed non-neutrilizing, opsonizing antibodies with combinatorial bactericidal activity of antibodies and

SUBMITTER: Singh R 

PROVIDER: S-EPMC5622086 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature