Direct modulation of T-box riboswitch-controlled transcription by protein synthesis inhibitors.

ABSTRACT: Recently, it was discovered that exposure to mainstream antibiotics activate numerous bacterial riboregulators that control antibiotic resistance genes including metabolite-binding riboswitches and other transcription attenuators. However, the effects of commonly used antibiotics, many of which exhibit RNA-binding properties, on the widespread T-box riboswitches, remain unknown. In Staphylococcus aureus, a species-specific glyS T-box controls the supply of glycine for both ribosomal translation and cell wall synthesis, making it a promising target for next-generation antimicrobials. Here, we report that specific protein synthesis inhibitors could either significantly increase T-box-mediated transcription antitermination, while other compounds could suppress it, both in vitro and in vivo. I

SUBMITTER: Stamatopoulou V 

PROVIDER: S-EPMC5622331 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature