β-catenin-mediated YAP signaling promotes human glioma growth.

ABSTRACT:

Background

Hippo/YAP pathway is known to be important for development, growth and organogenesis, and dysregulation of this pathway leads to tumor progression.We and others find that YAP is up-regulated in human gliomas and associated with worse prognosis of patients. However, the role and mechanism of YAP in glioma progression is largely unknown.

Methods

The expression of YAP in glioma tissues was detected by quantitative polymerase chain reaction (qPCR) and immunoblotting. The effect of YAP on glioma progression was examined using cell growth assays and intracranial glioma model. The effect of YAP on β-catenin protein level, subcellular location and transcription activity was examined by immunoblotting, immunofluorescence and RT-PCR.

Results

Firstly, knockdown of YAP

SUBMITTER: Wang Y 

PROVIDER: S-EPMC5622484 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature