Lineage- and developmental stage-specific mechanomodulation of induced pluripotent stem cell differentiation.

ABSTRACT:

Background

To maximize the translational utility of human induced pluripotent stem cells (iPSCs), the ability to precisely modulate the differentiation of iPSCs to target phenotypes is critical. Although the effects of the physical cell niche on stem cell differentiation are well documented, current approaches to direct step-wise differentiation of iPSCs have been typically limited to the optimization of soluble factors. In this regard, we investigated how temporally varied substrate stiffness affects the step-wise differentiation of iPSCs towards various lineages/phenotypes.

Methods

Electrospun nanofibrous substrates with different reduced Young's modulus were utilized to subject cells to different mechanical environments during the differentiation process towards represent

SUBMITTER: Maldonado M 

PROVIDER: S-EPMC5622562 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature