FMISO accumulation in tumor is dependent on glutathione conjugation capacity in addition to hypoxic state.

ABSTRACT:

Objective

¹⁸F-fluoromisonidazole (FMISO), a well-known PET imaging probe for diagnosis of hypoxia, is believed to accumulate in hypoxic cells via covalent binding with macromolecules after reduction of the nitro group. Previously, we showed the majority of ¹⁸F-FMISO was incorporated into low-molecular-weight metabolites in hypoxic tumors, and the glutathione conjugate of reduced FMISO (amino-FMISO-GS) distributed in the tumor hypoxic regions as revealed by imaging mass spectrometry (IMS). The present study was conducted to clarify whether FMISO is metabolized to amino-FMISO-GS within tumor cells and how amino-FMISO-GS contributes to FMISO accumulation in hypoxic cells. We also evaluated the relationship between FMISO accumulation and the glutathione conjugatio

SUBMITTER: Masaki Y 

PROVIDER: S-EPMC5622914 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature