De Novo Assembly of Transcriptome and Development of Novel EST-SSR Markers in Rhododendron rex Levl. through Illumina Sequencing.

ABSTRACT: Transcriptome sequences generated by next-generation sequencing (NGS) technologies can be utilized to rapidly detect and characterize a large number of gene-based microsatellites from different plants. Rhododendron rex Lévl. is a perennial woody species from the family Ericaceae and an endangered plant with high ornamental value endemic to Southwestern China. Nevertheless, the genetic and genomic information of R. rex remain unknown. In this study, we performed transcriptome sequencing for R. rex leaf samples, and generated large transcript sequences for functional characterization and development gene-associated SSR markers. A total of 164,242 unigenes were assembled and 115,089 (70.07%) unigenes were successfully annotated in public databases. In addition, a total of

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC5622969 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature