Identification and Characterization of Odorant Binding Proteins in the Forelegs of Adelphocoris lineolatus (Goeze).

ABSTRACT: The chemosensory system is essential for insects to detect exogenous compounds, and odorant binding proteins (OBPs) play crucial roles in odorant binding and transduction. In the alfalfa plant bug Adelphocoris lineolatus, an important pest of multiple crops, our understanding of the physiological roles of antenna-biased OBPs has increased dramatically, whereas OBPs related to gustation have remained mostly unexplored. In this study, we employed RNA sequencing and RACE PCR methods to identify putative OBPs from the adult forelegs of both sexes. Eight candidate OBPs were identified, and three OBPs (AlinOBP15, 16, and 17) were novel. Full-length sequence alignment and phylogenetic analyses suggested that these three candidate OBPs had characteristics typical of the insect OBP family. A

SUBMITTER: Sun L 

PROVIDER: S-EPMC5623005 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature