Loss of Nrf2 promotes alveolar type 2 cell loss in irradiated, fibrotic lung.

ABSTRACT: The development of radiation-induced pulmonary fibrosis represents a critical clinical issue limiting delivery of therapeutic doses of radiation to non-small cell lung cancer. Identification of the cell types whose injury initiates a fibrotic response and the underlying biological factors that govern that response are needed for developing strategies that prevent or mitigate fibrosis. C57BL/6 mice (wild type, Nrf2 null, Nrf2^flox/flox, and Nrf2^Δ/Δ; SPC-Cre) were administered a thoracic dose of 12Gy and allowed to recover for 250 days. Whole slide digital and confocal microscopy imaging of H&E, Masson's trichrome and immunostaining were used to assess tissue remodeling, collagen deposition and cell renewal/mobilization during the regenerative process. Histological asses

SUBMITTER: Traver G 

PROVIDER: S-EPMC5623074 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature