Intestinal Metabolites Are Profoundly Altered in the Context of HLA-B27 Expression and Functionally Modulate Disease in a Rat Model of Spondyloarthritis.

ABSTRACT:

Objective

HLA-B27-associated spondyloarthritides are associated with an altered intestinal microbiota and bowel inflammation. We undertook this study to identify HLA-B27-dependent changes in both host and microbial metabolites in the HLA-B27/β₂ -microglobulin (β₂ m)-transgenic rat and to determine whether microbiota-derived metabolites could impact disease in this major model of spondyloarthritis.

Methods

Cecal contents were collected from Fischer 344 33-3 HLA-B27/β₂ m-transgenic rats and wild-type controls at 6 weeks (before disease) and 16 weeks (with active bowel inflammation). Metabolomic profiling was performed by high-throughput gas and liquid chromatography-based mass spectrometry. HLA-B27/β₂ m-transgenic rats were treated w

SUBMITTER: Asquith M 

PROVIDER: S-EPMC5623151 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature