Resveratrol inhibits phorbol ester-induced membrane translocation of presynaptic Munc13-1.

ABSTRACT:

Background

Resveratrol (1) is a naturally occurring polyphenol that has been implicated in neuroprotection. One of resveratrol's several biological targets is Ca²⁺-sensitive protein kinase C alpha (PKCα). Resveratrol inhibits PKCα by binding to its activator-binding C1 domain. Munc13-1 is a C1 domain-containing Ca²⁺-sensitive SNARE complex protein essential for vesicle priming and neurotransmitter release.

Methods

To test if resveratrol could also bind and inhibit Munc13-1, we studied the interaction of resveratrol and its derivatives, (E)-1,3-dimethoxy-5-(4-methoxystyryl)benzene, (E)-5,5'-(ethene-1,2-diyl)bis(benzene-1,2,3-triol), (E)-1,2-bis(3,4,5-trimethoxyphenyl)ethane, and (E)-5-(4-(hexadecyloxy)-3,5-dihydroxystyryl)benzene-1,2,3-triol with Munc13-

SUBMITTER: Pany S 

PROVIDER: S-EPMC5623624 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature