β-Amyrin synthase from Conyza blinii expressed in Saccharomyces cerevisiae.

ABSTRACT: Conyza blinii H.Lév. is a widely used medicinal herb in southwestern China. The main pharmacological components of C. blinii are a class of oleanane-type pentacyclic triterpene glycosides known as conyzasaponins, which are thought to be synthesized from β-amyrin. However, no genes involved in the conyzasaponin pathway have previously been identified. Here, we identify an oxidosqualene cyclase (OSC), a β-amyrin synthase, which mediates cyclization of 2,3-oxidosqualene to yield β-amyrin. Ten OSC sequences were isolated from C. blinii transcript tags. Phylogenetic analysis was used to select the tag Cb18076 as the putative β-amyrin synthase, named CbβAS. The open reading frame of CbβAS is 2286 bp and encodes 761 amino acids. Its mature protein contains the h

SUBMITTER: Sun R 

PROVIDER: S-EPMC5623702 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature