Ammonia Oxidation and Nitrite Reduction in the Verrucomicrobial Methanotroph <i>Methylacidiphilum fumariolicum</i> SolV.

ABSTRACT: The Solfatara volcano near Naples (Italy), the origin of the recently discovered verrucomicrobial methanotroph Methylacidiphilum fumariolicum SolV was shown to contain ammonium ([Formula: see text]) at concentrations ranging from 1 to 28 mM. Ammonia (NH₃) can be converted to toxic hydroxylamine (NH₂OH) by the particulate methane monooxygenase (pMMO), the first enzyme of the methane (CH₄) oxidation pathway. Methanotrophs rapidly detoxify the intermediate NH₂OH. Here, we show that strain SolV performs ammonium oxidation to nitrite at a rate of 48.2 nmol [Formula: see text].h^-1.mg DW^-1 under O₂ limitation in a continuous culture grown on hydrogen (H₂) as an electron donor. In addition, strain SolV c

SUBMITTER: Mohammadi SS 

PROVIDER: S-EPMC5623727 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature