Pre-initiation complex assembly functions as a molecular switch that splits the Mcm2-7 double hexamer.

ABSTRACT: Initiation of chromosomal DNA replication in eukaryotes involves two steps: licensing and firing. In licensing, a core component of the replicative helicase, the Mcm2-7 complex, is loaded onto replication origins as an inactive double hexamer, which is activated in the firing step by firing factors. A reaction intermediate called the pre-initiation complex (pre-IC) has been proposed to assemble transiently during firing, but the existence of the pre-IC has not yet been confirmed. Here, we show, by systematic chromatin immunoprecipitation, that a distinct intermediate that fits the definition of the pre-IC assembles during firing in the budding yeast Saccharomyces cerevisiae Pre-IC assembly is observed in the absence of Mcm10, one of the firing factors, and is mutually dependent on a

SUBMITTER: Miyazawa-Onami M 

PROVIDER: S-EPMC5623835 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature