Embryonic stem cell-like cells established by culture of adult ovarian cells in mice.

ABSTRACT:

Objective

To suggest an alternative strategy for deriving histocompatible stems cells without undertaking genetic manipulation.

Design

Prospective approach using an animal model.

Setting

Stem cell and bioevaluation laboratory, Seoul National University.

Animal(s)

F1 (C57BL6 X DBA2) and outbred (ICR) mice.

Intervention(s)

Ovarian stroma cells of less than 40 mum in diameter were subcultured with fibroblast monolayer, and colony-forming cells were characterized.

Main outcome measure(s)

Stemness, genotype, and imprinted gene methylation.

Result(s)

Two-lines of colony-forming cells were established, which expressed markers specific for embryonic stem cells (ESC) and formed embryoid bodies and teratomas. Complete matching of microsatellite marke

SUBMITTER: Gong SP 

PROVIDER: S-EPMC5624318 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature