Sequential PET estimation of cerebral oxygen metabolism with spontaneous respiration of ¹⁵O-gas in mice with bilateral common carotid artery stenosis.

ABSTRACT: Positron emission tomography with ¹⁵O-labeled gases (¹⁵O-PET) is important for in vivo measurement of cerebral oxygen metabolism both in clinical and basic settings. However, there are currently no reports concerning ¹⁵O-PET in mice. Here, we developed an ¹⁵O-PET method applicable to mice with spontaneous respiration of ¹⁵O-gas without a tracheotomy catheter. Sequential ¹⁵O-PET was also performed in a mouse model of chronic cerebral hypoperfusion with bilateral common carotid artery stenosis (BCAS) induced by placement of microcoils. ¹⁵O-gas with isoflurane was supplied to the nose of mouse with evacuation of excess ¹⁵O-gas surrounding the body. ¹⁵O-PET was performed on days 3, 7, 14, 21, and

SUBMITTER: Temma T 

PROVIDER: S-EPMC5624393 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature