Stem Cell Differentiation as a Non-Markov Stochastic Process.

ABSTRACT: Pluripotent stem cells can self-renew in culture and differentiate along all somatic lineages in vivo. While much is known about the molecular basis of pluripotency, the mechanisms of differentiation remain unclear. Here, we profile individual mouse embryonic stem cells as they progress along the neuronal lineage. We observe that cells pass from the pluripotent state to the neuronal state via an intermediate epiblast-like state. However, analysis of the rate at which cells enter and exit these observed cell states using a hidden Markov model indicates the presence of a chain of unobserved molecular states that each cell transits through stochastically in sequence. This chain of hidden states allows individual cells to record their position on the differentiation trajectory, thereby encodin

SUBMITTER: Stumpf PS 

PROVIDER: S-EPMC5624514 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature