Estimating Human Trabecular Meshwork Stiffness by Numerical Modeling and Advanced OCT Imaging.

ABSTRACT:

Purpose

The purpose of this study was to estimate human trabecular meshwork (hTM) stiffness, thought to be elevated in glaucoma, using a novel indirect approach, and to compare results with direct en face atomic force microscopy (AFM) measurements.

Methods

Postmortem human eyes were perfused to measure outflow facility and identify high- and low-flow regions (HF, LF) by tracer. Optical coherence tomography (OCT) images were obtained as Schlemm's canal luminal pressure was directly manipulated. TM stiffness was deduced by an inverse finite element modeling (FEM) approach. A series of AFM forcemaps was acquired along a line traversing the anterior angle on a radially cut flat-mount corneoscleral wedge with TM facing upward.

Results

The elastic modulus of normal hTM esti

SUBMITTER: Wang K 

PROVIDER: S-EPMC5624775 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature