The Prohormone VGF Regulates β Cell Function via Insulin Secretory Granule Biogenesis.

ABSTRACT: The prohormone VGF is expressed in neuroendocrine and endocrine tissues and regulates nutrient and energy status both centrally and peripherally. We and others have shown that VGF-derived peptides have direct action on the islet β cell as secretagogues and cytoprotective agents; however, the endogenous function of VGF in the β cell has not been described. Here, we demonstrate that VGF regulates secretory granule formation. VGF loss-of-function studies in both isolated islets and conditional knockout mice reveal a profound decrease in stimulus-coupled insulin secretion. Moreover, VGF is necessary to facilitate efficient exit of granule cargo from the trans-Golgi network and proinsulin processing. It also functions to replenish insulin granule stores following nutrient stimulation. Our data

SUBMITTER: Stephens SB 

PROVIDER: S-EPMC5624795 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature