Early assessment of dosimetric and biological differences of total marrow irradiation versus total body irradiation in rodents.

ABSTRACT:

Purpose

To develop a murine total marrow irradiation (TMI) model in comparison with the total body irradiation (TBI) model.

Materials and methods

Myeloablative TMI and TBI were administered in mice using a custom jig, and the dosimetric differences between TBI and TMI were evaluated. The early effects of TBI/TMI on bone marrow (BM) and organs were evaluated using histology, FDG-PET, and cytokine production. TMI and TBI with and without cyclophosphamide (Cy) were evaluated for donor cell engraftment and tissue damage early after allogeneic hematopoietic cell transplantation (HCT). Stromal derived factor-1 (SDF-1) expression was evaluated.

Results

TMI resulted in similar dose exposure to bone and 50% reduction in dose to bystander organs. BM histology was similar betwee

SUBMITTER: Hui S 

PROVIDER: S-EPMC5624834 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature