Selective Homogeneous Assay for Circulating Endopeptidase Fibroblast Activation Protein (FAP).

ABSTRACT: Fibroblast Activation Protein (FAP) is a membrane-bound serine protease whose expression is often elevated in activated fibroblasts associated with tissue remodeling in various common diseases such as cancer, arthritis and fibrosis. Like the closely related dipeptidyl peptidase DPPIV, the extracellular domain of FAP can be released into circulation as a functional enzyme, and limited studies suggest that the circulating level of FAP correlates with the degree of tissue fibrosis. Here we describe a novel homogeneous fluorescence intensity assay for circulating FAP activity based on a recently identified natural substrate, FGF21. This assay is unique in that it can effectively distinguish endopeptidase activity of FAP from that of other related enzymes such as prolyl endopeptidase (PREP) and

SUBMITTER: Bainbridge TW 

PROVIDER: S-EPMC5624913 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature