Bioelectronic measurement and feedback control of molecules in living cells.

ABSTRACT: We describe an electrochemical measurement technique that enables bioelectronic measurements of reporter proteins in living cells as an alternative to traditional optical fluorescence. Using electronically programmable microfluidics, the measurement is in turn used to control the concentration of an inducer input that regulates production of the protein from a genetic promoter. The resulting bioelectronic and microfluidic negative-feedback loop then serves to regulate the concentration of the protein in the cell. We show measurements wherein a user-programmable set-point precisely alters the protein concentration in the cell with feedback-loop parameters affecting the dynamics of the closed-loop response in a predictable fashion. Our work does not require expensive optical fluorescence mea

SUBMITTER: Banerjee A 

PROVIDER: S-EPMC5624954 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature