Compound-specific isotope analysis resolves the dietary origin of docosahexaenoic acid in the mouse brain.

ABSTRACT: DHA (22:6n-3) may be derived from two dietary sources, preformed dietary DHA or through synthesis from α-linolenic acid (ALA; 18:3n-3). However, conventional methods cannot distinguish between DHA derived from either source without the use of costly labeled tracers. In the present study, we demonstrate the proof-of-concept that compound-specific isotope analysis (CSIA) by GC-isotope ratio mass spectrometry (IRMS) can differentiate between sources of brain DHA based on differences in natural ¹³C enrichment. Mice were fed diets containing either purified ALA or DHA as the sole n-3 PUFA. Extracted lipids were analyzed by CSIA for natural abundance ¹³C enrichment. Brain DHA from DHA-fed mice was significantly more enriched (-23.32‰ to -21.92‰) compared with mice on the AL

SUBMITTER: Lacombe RJS 

PROVIDER: S-EPMC5625118 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature