Near-infrared luminescent metallacrowns for combined in vitro cell fixation and counter staining.

ABSTRACT: Cell fixation is an essential approach for preserving cell morphology, allowing the targeting and labelling of biomolecules with fluorescent probes. One of the key requirements for more efficient fluorescent labelling is the preservation of cell morphology, which usually requires a combination of several fixation techniques. In addition, the use of a counter stain is often essential to improve the contrast of the fluorescent probes. Current agents possess significant limitations, such as low resistance toward photobleaching and sensitivity to changes in the microenvironment. Luminescent Ln³⁺ 'encapsulated sandwich' metallacrowns (MCs) overcome these drawbacks and offer complementary advantages. In particular, they emit sharp emission bands, possess a large difference between exc

SUBMITTER: Martinic I 

PROVIDER: S-EPMC5625569 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature