Screening and identification of host proteins interacting with Toxoplasma gondii SAG2 by yeast two-hybrid assay.

ABSTRACT:

Background

The identification of receptors or binding partners of Toxoplasma gondii from humans is an essential activity. Many proteins involved in T. gondii invasion have been characterized, and their contribution for parasite entry has been proposed. However, their molecular interactions remain unclear.

Results

Yeast two-hybrid (Y2H) experiment was used to identify the binding partners of surface antigens of T. gondii by using SAG2 as bait. Colony PCR was performed and positive clones were sent for sequencing to confirm their identity. The yeast plasmids for true positive clones were rescued by transformation into E. coli TOP 10F' cells. The interplay between bait and prey was confirmed by β-galactosidase assay and co-immunoprecipitation experiment. We detected 20 clones i

SUBMITTER: Lai MY 

PROVIDER: S-EPMC5625703 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature