O-5S quantitative real-time PCR: a new diagnostic tool for laboratory confirmation of human onchocerciasis.

ABSTRACT:

Background

Onchocerciasis is a parasitic disease caused by the filarial nematode Onchocerca volvulus. In endemic areas, the diagnosis is commonly confirmed by microscopic examination of skin snip samples, though this technique is considered to have low sensitivity. The available melting-curve based quantitative real-time PCR (qPCR) using degenerated primers targeting the O-150 repeat of O. volvulus was considered insufficient for confirming the individual diagnosis, especially in elimination studies. This study aimed to improve detection of O. volvulus DNA in clinical samples through the development of a highly sensitive qPCR assay.

Methods

A novel hydrolysis probe based qPCR assay was designed targeting the specific sequence of the O. volvulus O-5S rRNA gene. A total of 200

SUBMITTER: Mekonnen SA 

PROVIDER: S-EPMC5625774 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature