Crosstalk between glial and glioblastoma cells triggers the "go-or-grow" phenotype of tumor cells.

ABSTRACT:

Background

Glioblastoma (GBM), the most malignant primary brain tumor, leads to poor and unpredictable clinical outcomes. Recent studies showed the tumor microenvironment has a critical role in regulating tumor growth by establishing a complex network of interactions with tumor cells. In this context, we investigated how GBM cells modulate resident glial cells, particularly their paracrine activity, and how this modulation can influence back on the malignant phenotype of GBM cells.

Methods

Conditioned media (CM) of primary mouse glial cultures unexposed (unprimed) or exposed (primed) to the secretome of GL261 GBM cells were analyzed by proteomic analysis. Additionally, these CM were used in GBM cells to evaluate their impact in glioma cell viability, migration capacity and a

SUBMITTER: Oliveira AI 

PROVIDER: S-EPMC5625790 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature