Mechanosensing drives acuity of αβ T-cell recognition.

ABSTRACT: T lymphocytes use surface [Formula: see text] T-cell receptors (TCRs) to recognize peptides bound to MHC molecules (pMHCs) on antigen-presenting cells (APCs). How the exquisite specificity of high-avidity T cells is achieved is unknown but essential, given the paucity of foreign pMHC ligands relative to the ubiquitous self-pMHC array on an APC. Using optical traps, we determine physicochemical triggering thresholds based on load and force direction. Strikingly, chemical thresholds in the absence of external load require orders of magnitude higher pMHC numbers than observed physiologically. In contrast, force applied in the shear direction ([Formula: see text]10 pN per TCR molecule) triggers T-cell Ca²⁺ flux with as few as two pMHC molecules at the interacting surface interface w

SUBMITTER: Feng Y 

PROVIDER: S-EPMC5625899 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature