In vivo and in vitro protein imaging in thermophilic archaea by exploiting a novel protein tag.

ABSTRACT: Protein imaging, allowing a wide variety of biological studies both in vitro and in vivo, is of great importance in modern biology. Protein and peptide tags fused to proteins of interest provide the opportunity to elucidate protein location and functions, detect protein-protein interactions, and measure protein activity and kinetics in living cells. Whereas several tags are suitable for protein imaging in mesophilic organisms, the application of this approach to microorganisms living at high temperature has lagged behind. Archaea provide an excellent and unique model for understanding basic cell biology mechanisms. Here, we present the development of a toolkit for protein imaging in the hyperthermophilic archaeon Sulfolobus islandicus. The system relies on a thermostable protein tag (H5) c

SUBMITTER: Visone V 

PROVIDER: S-EPMC5626487 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature