Hierarchical role for transcription factors and chromatin structure in genome organization along adipogenesis.

ABSTRACT: The three dimensional folding of mammalian genomes is cell type specific and difficult to alter suggesting that it is an important component of gene regulation. However, given the multitude of chromatin-associating factors, the mechanisms driving the colocalization of active chromosomal domains and the role of this organization in regulating the transcription program in adipocytes are not clear. Analysis of genome-wide chromosomal associations revealed cell type-specific spatial clustering of adipogenic genes in 3T3-L1 cells. Time course analysis demonstrated that the adipogenic 'hub', sampled by PPARγ and Lpin1, undergoes orchestrated reorganization during adipogenesis. Coupling the dynamics of genome architecture with multiple chromatin datasets indicated that among all the transcription

SUBMITTER: Sarusi Portuguez A 

PROVIDER: S-EPMC5626622 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature