High-resolution studies of hydride transfer in the ferredoxin:NADP⁺ reductase superfamily.

ABSTRACT: Ferredoxin: NADP⁺ reductase (FNR) is an FAD-containing enzyme best known for catalysing the transfer of electrons from ferredoxin (Fd) to NADP⁺ to make NADPH during photosynthesis. It is also the prototype for a broad enzyme superfamily, including the NADPH oxidases (NOXs) that all catalyse similar FAD-enabled electron transfers between NAD(P)H and one-electron carriers. Here, we define further mechanistic details of the NAD(P)H ⇌ FAD hydride-transfer step of the reaction based on spectroscopic studies and high-resolution (~ 1.5 Å) crystallographic views of the nicotinamide-flavin interaction in crystals of corn root FNR Tyr316Ser and Tyr316Ala variants soaked with either nicotinamide, NADP⁺ , or NADPH. The spectra obtained from FNR crystal complexes match

SUBMITTER: Kean KM 

PROVIDER: S-EPMC5626627 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature