Transcriptional Output Transiently Spikes Upon Mitotic Exit.

ABSTRACT: The pulsatile nature of gene activity has recently emerged as a general property of the transcriptional process. It has been shown that the frequency and amplitude of transcriptional bursts can be subjected to extrinsic regulation. Here we have investigated if these parameters were constant throughout the cell cycle using the single molecule RNA FISH technique. We found evidence of transcriptional spikes upon mitotic exit in three different human cell lines. Recording of cell growth prior to hybridization and immuno-RNA FISH analysis revealed that these spikes were short-lived and subsided before completion of cytokinesis. The transient post-mitotic increase in transcriptional output was found to be the result of cells displaying a higher number of active alleles and/or an increased number

SUBMITTER: Vankova Hausnerova V 

PROVIDER: S-EPMC5626720 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature