Mechanistic understanding of in vivo protein corona formation on polymeric nanoparticles and impact on pharmacokinetics.

ABSTRACT: In vitro incubation of nanomaterials with plasma offer insights on biological interactions, but cannot fully explain the in vivo fate of nanomaterials. Here, we use a library of polymer nanoparticles to show how physicochemical characteristics influence blood circulation and early distribution. For particles with different diameters, surface hydrophilicity appears to mediate early clearance. Densities above a critical value of approximately 20 poly(ethylene glycol) chains (MW 5 kDa) per 100 nm² prolong circulation times, irrespective of size. In knockout mice, clearance mechanisms are identified for nanoparticles with low and high steric protection. Studies in animals deficient in the C3 protein showed that complement activation could not explain differences in the clearance of

SUBMITTER: Bertrand N 

PROVIDER: S-EPMC5626760 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature