Mitotic post-translational modifications of histones promote chromatin compaction in vitro.

ABSTRACT: How eukaryotic chromosomes are compacted during mitosis has been a leading question in cell biology since the nineteenth century. Non-histone proteins such as condensin complexes contribute to chromosome shaping, but appear not to be necessary for mitotic chromatin compaction. Histone modifications are known to affect chromatin structure. As histones undergo major changes in their post-translational modifications during mitotic entry, we speculated that the spectrum of cell-cycle-specific histone modifications might contribute to chromosome compaction during mitosis. To test this hypothesis, we isolated core histones from interphase and mitotic cells and reconstituted chromatin with them. We used mass spectrometry to show that key post-translational modifications remained intact during our

SUBMITTER: Zhiteneva A 

PROVIDER: S-EPMC5627050 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature